Phosphatase activity during growth ofYarrowia lipolytica
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چکیده
منابع مشابه
Effect of copper on acid phosphatase activity in yeast Yarrowia lipolytica.
Acid phosphatase (APase) activity of the yeast Yarrowia lipolytica increased with increasing Cu2+ concentrations in the medium. Furthermore, the enzyme in soluble form was stimulated in vitro by Cu2+, Co2+, Ni2+, Mn2+ and Mg2+ and inhibited by Ag+ and Cd2+. The most effective ion was Cu2+, especially for the enzyme from cultures in medium containing Cu2+, whereas APase activity in wall-bound fr...
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Studies on the nucleolytic activity of cell homogenates of Tetrahymena pyriformis indicated that enzyme activity was, among other factors, a function of the age of the culture (1, 2). It was shown that the specific activities of ribonuclease (RNase) (EC 2.7.7.h) and acid phosphatase (APase) (EC 3.1.3.2) in crude homogenates prepared from stationary-phase cultures were consistently higher than t...
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In a study on 857 infants born preterm, high peak plasma alkaline phosphatase activity was independently related to slower growth rate in the neonatal period, and to a highly significant reduction in attained length at 9 months and 18 months post term. At 18 months the deficit in body length associated with peak neonatal plasma alkaline phosphase activity of 1200 IU/l or more was 1.6 cm (95% co...
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We have cloned and characterized the gene PYC1, encoding the unique pyruvate carboxylase in the dimorphic yeast Yarrowia lipolytica. The protein putatively encoded by the cDNA has a length of 1,192 amino acids and shows around 70% identity with pyruvate carboxylases from other organisms. The corresponding genomic DNA possesses an intron of 269 bp located 133 bp downstream of the starting ATG. I...
متن کاملCopper extrusion after accumulation during growth of copper-tolerant yeast Yarrowia lipolytica.
The Cu2+-tolerant yeast Yarrowia lipolytica accumulated Cu2+ until the late logarithmic phase. Thereafter, Cu2+ was temperature-dependently extruded into phosphate-limited culture medium containing high concentrations of heavy metal ions but not into 10 mM 2-(N-morpholino)ethane sulfonic acid (MES) buffer (pH 6.0). Peptone in the culture medium played an important role in the extrusion, which p...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1993
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1993.tb06141.x